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Question

The acid used for preliminary separation of biomolecule in a living tissue is

A
trichlorobenzoic acid
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B
benzoic acid
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C
trichloroacetic acid
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D
acetic acid
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Solution

The correct option is C trichloroacetic acid
The living tissue is grinded using a mortar and pestle. The living tissue is mixed with trichloroacetic acid while grinding. The solution obtained is then filtered and separated into two fractions - acid soluble pool and acid insoluble pool.
The acid soluble pool consists of molecules whose molecular weight between 18 to 800 daltons. They are called micromolecules. Whereas molecules in the acid insoluble pool consist of molecules with a molecular weight of 800 daltons and above (with the exception of lipids). They are called macromolecules.

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