CameraIcon
CameraIcon
SearchIcon
MyQuestionIcon


Question

The specific DNA sequence where EcoRl cuts is


A
ATTCGA
TAAGCT
loader
B
GAATTC
CTTAAG
loader
C
GCTTAA
CGAATT
loader
D
GTTCAA
CAAGTT
loader
E
TTCGAA
AAGCTT
loader

Solution

The correct option is B GAATTC
CTTAAG
Restriction enzymes like EcoRI, cut through the DNA strands at nucleotides that are not exactly opposite each other. This creates DNA fragments with one nucleotide strand that overhangs at the end. This overhanging nucleotide strand is called as a sticky end because it can easily bond with complementary DNA fragments. It creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry.
Thus, the correct answer is option B.

Biology

Suggest Corrections
thumbs-up
 
0


similar_icon
Similar questions
View More


similar_icon
People also searched for
View More



footer-image