Question

Thermal cycler is used in the reaction is

• A. Radioacivity
• B. Enzyme catalyzed reaction
• C. Chemical reaction
• D. Polymerase chain reaction

Answer 1

The correct option is D Polymerase chain reaction

Polymerase chain reaction is a technique which is mainly used to amplify a DNA segment. It was given by Kary Mullis. The sample DNA strand is denatured by heating at the temperature of 95${}^o$C. Each strand act as a template for the new strand. The primers are annealed at 55 ${}^o$C to all the template. In the third step, the temperature is raised to about 72${}^o$C and the DNA polymerase also known as Taq polymerase begins adding nucleotides onto the ends of the annealed primers.

The thermal cycler is an instrument with microprocessor-controlled temperature cycling required for polymerase chain reaction. It is a technique discovered in 1975 for recombinant DNA and gene cloning. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then rises and lowers the temperature of the block in discrete, pre-programmed steps.

So, the correct answer is option D.