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Recombinant DNA Technology

Using recombi...

Question

Using recombinant DNA technology, genes from a donor cell can be implanted into a bacterium for DNA replication and protein synthesis. The kind of cells that can be used as gene donors are

A

only bacteria

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B

only eukaryotic cells

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C

only yeast cells

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D

any kind of cells

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Solution

The correct option is D any kind of cells
Recombinant DNA technology involves joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations. DNA molecules as a source of genes can be obtained from bacteria, yeast, animals, humans or any other type of cells.
So, the correct option is 'any kind of cells'.

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Q. Using recombinant DNA technology, genes from a donor cell can be inserted into a bacterium for DNA replication and protein synthesis. The kind of cells that can be used as gene donors in this technology are

Q. An advantage of using yeasts rather than bacteria as recipient cells for the recombinant DNA of eukaryotes is that yeasts can ______________.

Q. Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombination DNA technology.

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
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III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
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Q. Study the following statements regarding recombinant DNA technology and select the incorrect ones

(i) Taq polymerase extends the primers using the nucleotides provided in the reaction

(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology

(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis

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