Question

Which blotting is used in DNA Fingerprinting?

Answer 1

DNA fingerprinting:

Based on the nucleotide sequences of certain areas of human DNA that are exclusive to each person, DNA fingerprinting is a laboratory method used to ascertain a person's likely identification.

Southern Blotting:

1. Southern blotting is a test commonly used in molecular biology and genetics.
2. The purpose of the test is to check for a match between DNA molecules.

Procedure/Step:

1. Extraction and purification of DNA from cells
2. Restriction Digestion of DNA Fragmentation.
3. Separation by Electrophoresis
4. Depurination
5. Denaturation
6. Blotting and Baking
7. Hybridization
8. Washing of unbound probes
9. Autoradiograph

Application:

1. To identify a match between specific DNA samples
2. To Isolate the desired DNA for construction of the rDNA.
3. Identification of criminal
4. Used in the diagnosis of cancer.
5. Diagnosis of HIV and any other genetic diseases
6. Paternity test