Question

Which of the following is commonly used as a vector before introducing a DNA fragment in human lymphocytes?

1. Retrovirus

2. lambda phage

3. Ti plasmid

4. pBr 322

Answer 1

1.
Retrovirus is commonly used as vector for introducing a DNA fragment in human lymphocyte.
Gene therapy : Lymphocyte from blood of patient are grown in culture outside the body, a functional gene is introduced by using a retroviral vector, into these lymphocyte.