The correct option is B Study of chromosome - Metaphase
Among all the phases, metaphase is the best phase to observe the shape, number and size of the chromosomes. The chromosomes align themselves along the imaginary equatorial plane during metaphase.
The nuclear membrane completely disappears at the end of prophase.
In metaphase I stage, the protein fibres formed in between the centromeres of homologous chromosome are called interzonal fibres.
During telophase, the nucleolus, ER and Golgi apparatus reappears.