Question

Which of the following is not a characteristic of pBR322 vector?

• A. It was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez
• B. It is the most widely used, versatile and easily manipulated vector
• C. It has two antibiotic resistance genes $te{t}^R$ and $am{p}^R$
• D. It does not have restriction site for SaII

Answer 1

The correct option is D It does not have restriction site for SaII

pBR322 was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez. It is widely used in gene cloning experiments. In pBR322 plasmid vector, p - denotes that it is a plasmid; BR - stands for Boliver and Rodriguez who constructed this plasmid; 322 - Is a number given to distinguish this plasmid from others developed in the same laboratory. E coli cloning vector pBR 322 has restriction sites {HindIII, EcoRI, BamHl, SaII, PvuII, PstI, ClaI), ori(origin of replication) and antibiotic resistance genes ($am{p}^R$ and $te{t}^R$). rop codes for the proteins involved in the replication of the ] plasmid.