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Question

Which of the following is the true reference to transformation in bacteria?
(I) Transfer of genetic material is mediated by 'F' factor.
(II) Uptake of naked DNA fragments from surrounding environment and the expression of that genetic information in the recipient cell.
(III) Transformation experiments were the basis to propose the chromosomal theory of inheritance.
(IV) Transformation was discovered by Frederick Griffith in Streptococcus pneumoniae.

A
I, IV
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B
II, III, IV
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C
III, IV
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D
II, IV
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Solution

The correct option is A I, IV
I) F factor is known as fertility factor. During the conjugation, the donor bacteria transfers its genetic material to the recipient through direct contact. It allows genes to be transferred from one bacterium carrying the factor to another bacterium lacking the factor by conjugation.
II) Uptake of naked DNA fragments doesn't take from the surrounding environment automatically rather it is transferred through self-replicating DNA molecule known as the plasmid and the expression of the genetic information in the recipient cell.
III) Transformation experiments were the basis to propose that DNA is the genetic material which passes from parents to offspring.
IV) The transformation was discovered by Frederick Griffith in Streptococcus pneumoniae. transformation is one of three processes for horizontal gene transfer, in which exogenous genetic material passes from bacterium to another.
So, the correct answer is option A.

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