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Question

Which of the following steps is catalysed by Taq polymerase?


A
Denaturation of template DNA
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B
Annealing of primers to template DNA
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C
Extension of primer end on the template DNA
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D
Maintainence of high temperature during PCR
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Solution

Taq polymerase is used in polymerase chain reaction (PCR).

PCR is a method of amplifying DNA sequences. There are several steps involved in the process:
  1. Denaturation: The two strands of the DNA are separated due to high temperature (94οC)
  2. Annealing: Primers are annealed to the template DNA strands to initiate replication process
  3. Extension: DNA polymerisation and extension of the primer end on the template strands of the DNA using Taq polymerase happen in this step
Taq polymerase is a DNA-dependent DNA polymerase which is unique in carrying out DNA polymerisation reaction at high temperatures (thermostable polymerase).

Biology

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