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Selection Markers

Why is it ess...

Question

Why is it essential to have selectable marker in a cloning vector?

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Solution

Selectable markers are essential to identify and eliminate non-transformants (no recombinant DNA), and selectively permitting the growth of the transformants (host cells bearing recombinant DNA).

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Similar questions

Q. Name the selectable markers in the cloning vector pBR322?

Q. Which of the following are essential features of a cloning vector used to create transgenic organisms:
1.Presence of ori site
2.Presence of selectable markers
3.Presence of restriction endonuclease recognition sites

Q. What is significance of selectable marker in cloning vector?

Q. (a) Name the selectable markers in the cloning vector pBR322? Mention the role they play.
(b) Why is the coding sequence of an enzyme

β

-galactosidase, a preferred selectable marker in comparison to the ones named above?

Q. Name the selectable markers in the cloning vector

p B R 322

? Mention the role they play.

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