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Question

Widal test relies on the detection of
  1. Antibodies in the patient's blood serum
  2. S.Typhi specific antibodies in the patient's blood serum
  3. An antigen antibody complex in the patient's blood serum
  4. Inactivated Salmonella antigens in the patient's blood serum


Solution

The correct option is B S.Typhi specific antibodies in the patient's blood serum
Widal’s test is based on the principle that a patient suffering from typhoid has a lot of antibodies that will react against the antigens of Salmonella Typhi. So we take the blood serum of the patient and add it to a Widal test suspension that contains inactivated preparations of various Salmonella antigens. The antibodies in the patient's serum will bind to the antigens in the suspension and it appears as agglutination or aggregation of antibody-antigen complex. Therefore appearance of agglutination indicates a positive diagnosis for typhoid.

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