Restriction endonucleases are a class of enzyme that cut DNA molecules.
Restriction enzymes belong to a larger class of enzymes called
nucleases. These are of two kinds:
I. Exonucleases remove nucleotides from the ends of the DNA.
II. Endonucleases make cuts at specific positions within the DNA.
There are four classes of restriction endonucleases:
Types I, II,III and IV.
All
types of enzymes recognize specific short DNA sequences and carry out
the endonucleolytic cleavage of DNA to give specific double-stranded
fragments with terminal 5'-phosphates. They differ in their recognition
sequence, sub unit composition, cleavage position, and cofactor
requirements.
"Synthesis of recombinant DNA molecule is possible only
when the vector and source DNA is cut by the same restriction enzyme"
Explanation:
Restriction
enzymes cut the strand of DNA a little away from the centre of the
palindrome sites, but between the same two bases on the opposite
strands. This leaves single stranded portions at the ends. Them are
overhanging stretches called sticky ends on each strand. These
are named so because they form hydrogen bonds with their complementary
cut counterparts. This stickiness of the ends facilitates the action of
the enzyme DNA ligase. Restriction endonucleases are used in genetic
engineering to form 'recombinant' molecules of DNA, which are composed
of DNA from different sources/genomes. When cut by the same restriction
enzyme, the resultant DNA fragments have the same kind of 'sticky-ends'
and, these can be joined together (end-to-end) using DNA ligases.
Normally, unless one cuts the vector and the source DNA with the same
restriction enzyme, the recombinant vector molecule cannot be created.