a) Sterile conditions
b) Growth supporting medium
c) Hormone supplementation
d) All of the above
Answer: d) All of the above
Explanation: An explant is a piece of tissue or organ removed from a plant to culture new cells from it. A callus is a mass of unorganised cells. When an explant is provided with sterile conditions, proper medium and hormones for growth, it develops into a callus.
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