Sterilization of tissue culture medium is done by (A) Keeping the medium at -20°C. (B) Filtering the medium through a fine sieve. (C) Mixing the medium with antifungal agents. (D) Autoclaving of the medium at 120°C for 15 minutes.

The correct answer is (D) Autoclaving of the medium at 120°C for 15 minutes.

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