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Question

The principle involved in paper chromatography is ______.


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Solution

Chromatography

  • Chromatography is an important technique extensively used to separate mixtures into their components, purify compounds, and also to test the purity of compounds.

Paper chromatography

  • “Partition chromatography is based on the continuous differential partitioning of components of a mixture between stationary and mobile phases.”
  • Paper chromatography is a type of partition chromatography.
  • Chromatography paper is a unique kind of paper that is used in paper chromatography. Water that has been trapped inside a chromatography paper serves as the stationary phase.
  • A strip of chromatography paper that has been smeared at the base with the mixture's solution is suspended in an appropriate solvent or a combination of solvents. The mobile phase is this solvent.
  • Capillary action causes the solvent to ascend up the paper and run over the spot.
  • So, the principle involved in paper chromatography is partition.

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