The principle of paper chromatography is partition. In paper chromatography, there are two phases: the stationary phase and the other is the mobile phase. Here, water trapped in the paper acts as the stationary phase and solvent acts as the mobile phase.
Chromatography is used to separate mixtures of substances into their components. Paper chromatography (PC) is a type of chromatography where the procedures are run on specialized paper. It was first introduced by German scientist Christian Friedrich Schonbein (1865).
- Substances are distributed between a stationary phase and a mobile phase.
- Cellulose layers in filter paper contain moisture which acts as a stationary phase. Organic solvents/buffers are used as the mobile phase.
- The stationary phases carry the sample in developing the solution.
- Components of the sample will separate readily according to how strongly they adsorb onto the stationary phase versus how readily they dissolve in the mobile phase.