The lithosphere is the solid, outer part of the Earth, including the brittle upper portion of the mantle and the crust. The lithosphere is the coolest and most rigid part of the Earth. The biosphere is the life and ecology of a planet. It is not a distinct area, but rather a collection of areas, including parts of the atmosphere, lithosphere and hydrosphere, where organisms live and dead organic matter rests.The key difference between biosphere and lithosphere is that the biosphere is a part of the crust and atmosphere that support living matter while the lithosphere is the solid shell of the Earth that includes the crust and part of the uppermost mantle.