Bolivar and Rodriguez created pBR322 in Herbert Boyer’s laboratory at the University of California in 1977. To identify this plasmid from other vectors, they gave it the number 322. As a result, the ‘A’ assertion is correct. A typical plasmid cloning vector is the pBR322 DNA vector. As a result, option ‘B’ is also valid. It has genes for ampicillin and tetracycline resistance, which may be amplified using chloramphenicol and aid in the selection of recombinant cells from a mixed culture including both recombinant and non-recombinant cells. As a result, option ‘C’ is likewise a valid statement. Restriction sites in the pBR322 vector include Hindlll, EcoRI, BamHI, Sall, Pvill, Pstl, Clal, and ori (origin of replication).