Which of the following is not a characteristic of pBR322 vector? A. It was the first artificial cloning vector constructed in 1977 by Bolivar and Rodriguez B. It is the most widely used, versatile and easily manipulated vector. C. It has two antibiotic resistance genes tetR and ampR. D. It does not have restriction site for Sall.

Answer: (d)


Bolivar and Rodriguez created pBR322 in Herbert Boyer’s laboratory at the University of California in 1977. To identify this plasmid from other vectors, they gave it the number 322. As a result, the ‘A’ assertion is correct. A typical plasmid cloning vector is the pBR322 DNA vector. As a result, option ‘B’ is also valid. It has genes for ampicillin and tetracycline resistance, which may be amplified using chloramphenicol and aid in the selection of recombinant cells from a mixed culture including both recombinant and non-recombinant cells. As a result, option ‘C’ is likewise a valid statement. Restriction sites in the pBR322 vector include Hindlll, EcoRI, BamHI, Sall, Pvill, Pstl, Clal, and ori (origin of replication).

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