Write a short note on C-Terminal group analysis for amino acids.

Hydroxyl and carboxyl groups present in the peptide are to be first protected as amides or esters. Terminal carboxyl group is converted into an anhydride first and treated with iso-thiocyanate to give a C-terminal acyl thiocyanate peptide product. The product automatically rearranges to a thiohydantoin incorporating the penultimate C-terminal unit. Repetitive analyses may be conducted in much the same way they are with the Edman procedure to find the amino acid sequence in the chain.

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