# Algae Biofuel Process

## Objective

Algaes are the organisms which are commonly found in most of the aquatic environments. The large type of multicellular algaes reside in ponds and also in the oceans. Microalgaes are the tiniest unicellular organisms which develop as suspensions in the water and they can be measured in micrometers. The aim of this experiment is to measure the number of Calories present in an algae samples which are obtained from various environments and also to compare quantity of oil that is present in each of the sample.

## Requirements

• Food calorimeter
• Collection jars
• Algae
• Notebook
• Microwave oven
• Filter papers
• Thermometer

## Procedure

1. Build food calorimeter or even you can purchase it.
2. Collect different samples of algae from different sources like pond, ocean, marshes, or even swamps. Then, store these algae specimens in jar which is filled with some water.
3. Note conditions in the notebook as to from where you have got the samples
4. Also record as to these conditions that had placed a growth stress on these algae.
5. Make a hypothesis predicting as to which sample of algae contains more oil
6. Take off the water from specimen containing the algae by heating in the microwave oven. Make sure you do not burn any of the algae
7. Measure about equal quantities of each kind of dried algae
8. Later fill up the water chamber of the calorimeter up to calibration point. Then measure water temperature
9. Burn algae sample(any one) in the calorimeter chamber, thus allowing the heat which is produced by combustion in order to heat up the water.
10. Continue the process of burning till the samples gets reduced to carbon.
11. Then, measure temperature of water after the completion of burning up of algae
12. Compute the number of calories that is produced by the burning of algae.
13. Repeat this process for each of the samples of algae
14. Now, differentiate between the number of calories that is produced by burning up of each of samples of algae
15. Assess this hypothesis in the light of your experiment.

## Conclusion

Algaes require water, sunlight, nutrients along with carbon dioxide in order to grow. By photosynthesis process, algaes convert carbon dioxide into glucose. This glucose is broken down into some fatty acids which under the normal conditions are used for the production of membranes for the new algal cells. When these algaes are starved out of nutrients, then the fatty acids in them produce the fat molecules which is nothing but the oil or the fuel. Most of the algae does not produce much of oil unless & un-till they get physiologically stressed or say when they do not get their basic requirements which are necessary for the growth.

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