Biomolecules JEE Notes Pdf

Biomolecules JEE notes PDF helps students to have a quick revision before the entrance exam. We provide notes that help students to understand each topic in a better way. Biomolecules JEE notes PDF includes all the important definitions, facts and formulas from the topic biomolecules. Students are recommended to go through these notes so that they won’t miss any important point for the exam.

The topics in this article include characteristics of carbohydrates, proteins and nucleic acids and hormones, classification of carbohydrates, and vitamins on the basis of their structures, difference between DNA and RNA, and the role of biomolecules in the biosystem. Biomolecules are the organic compounds which form the basis of life. They build up the living system and are responsible for their growth and maintenance.

Table of Contents


Carbohydrates are defined as optically active polyhydroxy aldehydes or ketones or the compounds which produce such units on hydrolysis. Carbohydrates are also known as saccharides. According to their behaviour on hydrolysis, carbohydrates are classified into three groups. Monosaccharides, Oligosaccharides and Polysaccharides.

Monosaccharides are the simplest carbohydrates. These cannot be hydrolysed into simpler compounds. E.g. Glucose, ribose. Oligosaccharides are carbohydrates which give 2 to 10 monosaccharide units on hydrolysis. E.g. Sucrose, Lactose, Maltose. They are further classified as disaccharides, trisaccharides, tetrasaccharides, etc. Polysaccharides are carbohydrates which give a large number of monosaccharide units on hydrolysis. E.g. Cellulose, starch. Polysaccharides are not sweet in taste, hence they are also called non-sugars.


On the basis of the number of carbon atoms and the functional group, monosaccharides are again classified. If it contains a keto group, it is known as a ketose. If a monosaccharide contains an aldehyde group, it is known as an aldose.

Preparation of Glucose

Glucose is the monomer for many other carbohydrates. Glucose is probably the most abundant organic compound on the earth. Glucose occurs freely in nature as well as in the combined form. It is present in sweet fruits and honey. The molecular formula is C6H12O6.

a) from sucrose

C12H22O11 + H2O → C6H12O6 + C6H12O6

Sucrose Glucose Fructose

b) From starch

(C6H10O5)n + nH2O → nC6H12O6

Starch or cellulose Glucose

The cyclic structure of glucose is shown below.

Glucose Cyclic Structure


Fructose is obtained along with glucose by the hydrolysis of disaccharide, sucrose. It is found in fruits, honey and vegetables. It is a natural monosaccharide. It is used as a sweetener in its pure form. It is an important ketohexose. Fructose has molecular formula C6H12O6. It belongs to D-series and is a laevorotatory compound. It is appropriately written as D-(–)-fructose. The open chain structure is shown below.


The cyclic structures of two anomers of fructose (Haworth structures) are given below.

Anomers Of Fructose


Disaccharides yield two molecules of either the same or different monosaccharides, on hydrolysis with dilute acids or enzymes . The two monosaccharides are joined together by an oxide linkage formed by the loss of a water molecule. Such a linkage through oxygen an atom is called glycosidic linkage.

(a) Sucrose: It is one of the common disaccharides which on hydrolysis gives an equimolar mixture of D-(+)-glucose and D-(-) fructose.

C12H22O11+H2O → C6H12O6 + C6H12O6

By a glycosidic linkage between C1 of α-D-glucose and C2 of β-D-fructose, these two monosaccharides are held together.


(b) Maltose: It is composed of two α-D-glucose units in which C1 of one glucose (I) is linked to C4 of another glucose unit (II). The free aldehyde group can be produced at C1 of second glucose in solution. It exhibits reducing properties. So it is a reducing sugar.


(c) Lactose: It is also a reducing sugar. This disaccharide is found in milk. Hence, it is known as milk sugar. It is composed of β-D-galactose and β-D-glucose. The linkage is between C1 of galactose and C4 of glucose.



A large number of monosaccharide units joined together by glycosidic linkages to form polysaccharides. They mainly act as the food storage or structural materials.

(i) Starch: It is the main storage polysaccharide of plants and dietary source for human beings. High content of starch is found in cereals, roots, tubers and some vegetables. It is a polymer of α-glucose and consists of two components – Amylose and Amylopectin. Amylose is water soluble and Amylopectin is insoluble in water.

(ii) Cellulose: Cellulose occurs in plants and it is the most abundant organic substance in the plant kingdom.

(iii) Glycogen: The carbohydrates are stored in the animal body as glycogen. Glycogen is also called animal starch.


All proteins are polymers of α-amino acids. Main sources of proteins are milk, cheese, fish, meat, pulses, peanuts, etc. They form the fundamental basis of structure and functions of life. They are also required for growth and maintenance of the body.

Amino acids

Amino acids contain carboxyl (-COOH) and amino (-NH2 ) functional groups. According to the relative position of the amino group with respect to the carboxyl group, the amino acids can be classified as α, β, γ, δ and so on. On hydrolysis of proteins, only α-amino acids are obtained.

Alpha Amino Acid

Essential and non essential amino acids

Non-essential amino acids are amino acids which can be synthesised in the body. The amino acids which cannot be synthesised by the body are called essential amino acids. They must be obtained through diet.

Zwitter ion

In aqueous solution, the carboxyl group can lose a proton and an amino group can accept a proton, giving rise to a dipolar ion known as Zwitter ion.

Zwitter ion

On the basis of the molecular shape, proteins can be classified into fibrous proteins and globular proteins. Keratin and myosin are examples of fibrous proteins. They are insoluble in water. Insulin and albumin are examples of globular proteins. They are soluble in water.

Structure and shape of proteins can be studied at four different levels.

Primary structure: Proteins may have one or more polypeptide chains. Each polypeptide in a

protein has amino acids linked with each other in a specific sequence and it is this sequence of amino acids that is said to be the primary structure of that protein.

Secondary structure: The secondary structure of protein refers to the shape in which a long polypeptide chain can exist.

Tertiary structure: The tertiary structure of proteins represents overall folding of the polypeptide chains.

Quaternary structure: Some proteins are made up of two or more polypeptide chains known as sub-units. The spatial arrangement of these subunits with respect to each other is known as quaternary structure.

Denaturation of Proteins

The secondary or tertiary structure of proteins get disturbed on change of pH or temperature and they are not able to perform their functions. This is called denaturation of proteins. The coagulation of egg white on boiling and curdling of milk are examples.

Importance of Proteins

a) Hormones, such as insulin and glucagon are proteins.

b) Proteins participate in the growth and repair mechanism of body tissues.

c) A protein called fibrinogen helps to stop bleeding.

d) Oxygen is transported to different tissues from blood by haemoglobin which is a protein.

e) Proteins are structural components of cells.

f) The proteins known as immunoglobulins serve in defence against infections.


Various chemical reactions in living organisms involve a sequence of reactions and all these reactions occur in the body under very mild conditions. This occurs with the help of certain biocatalysts called enzymes. Maltase is the enzyme that catalyses hydrolysis of maltose into glucose.


These are organic compounds required in the diet in small amounts to perform specific biological functions for normal maintenance of optimum growth and health of the organism.

a) Fat soluble vitamins: These are soluble in fat and oils but insoluble in water are kept in this group. These are vitamins A, D, E and K.

b) Water soluble vitamins: B group vitamins and vitamin C are soluble in water and thus they are grouped together. Water soluble vitamins are readily excreted in urine and cannot be stored (except vitamin B12) in our body. So these vitamins must be supplied regularly in diet.

The following table shows vitamins, their deficiency diseases and the sources.

Vitamins Deficiency diseases Sources
Vitamin A Xerophthalmia, Night blindness Fish liver oil, carrots, butter and milk
Vitamin B1 (Thiamine) Beri beri Green vegetables, cereals, yeast, and milk.
Vitamin B2 (Riboflavin) Digestive disorders and burning sensation of the skin, Cheilosis Milk, eggwhite, liver, kidney
Vitamin B6 (Pyridoxine) Convulsions Yeast, milk, egg yolk, cereals and grams
Vitamin B12 Pernicious anaemia Meat, fish, egg and curd
Vitamin C (Ascorbic acid) Scurvy (bleeding gums) Citrus fruits, amla and green leafy vegetables
Vitamin D Rickets and osteomalacia Exposure to sunlight, fish and egg yolk
Vitamin E Increased fragility of RBCs and muscular weakness Vegetable oils like wheat germ oil, sunflower oil, etc.
Vitamin K Increased blood clotting time Green leafy vegetables

Nucleic acids

The particles in the nucleus of the cell, responsible for heredity, are called chromosomes which are made up of proteins and another type of biomolecules called nucleic acids. The two types of nucleic acids are DNA and RNA. In DNA molecules, the sugar is β-D-2-deoxyribose. In RNA molecule, it is β-D-ribose.

Nucleic Acid

DNA contains four bases.

  • Adenine (A)
  • Guanine (G)
  • Cytosine (C)
  • Thymine (T)

RNA also contains four bases, the first three bases are the same as in DNA but the fourth one is uracil (U). There are three types of RNA. They are mRNA, rRNA and tRNA which carry out the protein synthesis in the cell.

DNA is a double stranded molecule. RNA is a single stranded molecule.
DNA is the chemical basis of heredity. RNA helps in protein synthesis.
Adenine (A), Guanine (G), Cytosine (C)

Thymine (T) are the 4 bases.

Adenine (A), Guanine (G), Cytosine (C)

Uracil (U) are the 4 bases.

DNA contains deoxyribose. RNA contains ribose as sugar.


Hormones are produced by endocrine glands in the body and are poured directly in the blood which transports them to the site of action. They act as intercellular messengers. They help to maintain the balance of biological activities in the body.

Solved Examples

Question 1:

How is glucose related with fructose?

1) Functional group isomerism

2) Rotamers

3) Position isomerism

4) Geometrical isomerism


Glucose and fructose have the same molecular formula C6H12O6 but differ in the functional group having aldehydic and ketonic group, respectively. So, glucose and fructose are the functional group isomers.

Hence option 1 is the answer.

Question 2:

The sweetest carbohydrate is

1) sucrose

2) glucose

3) fructose

4) lactose


Fructose is the sweetest of all carbohydrates that occurs naturally.

Hence option 3 is the answer.

Question 3:

A carbohydrate is treated with α-naphthol and conc. H2SO4. What colour will be formed at the junction of two liquids?

1) Blood-red

2) Violet

3) Brown

4) Orange


Carbohydrate reacts with α-naphthol and conc. H2SO4 to give violet colour. This is Molisch’s test for carbohydrates.

Hence option 2 is the answer.

Question 4:

Milk changes after digestion into

1) cellulose

2) fructose

3) glucose

4) lactose


The main carbohydrate in milk is lactose. Before absorption lactose must be broken down into its two individual sugars (glucose and galactose). Lactose is broken down by the enzyme lactase that is secreted by the intestinal cells. So, milk changes into glucose and galactose after digestion.

Hence option 3 is the answer.

Question 5:

Cellulose is a polymer of

1) glucose

2) fructose

3) ribose

4) sucrose


Cellulose is an organic compound with the formula (C6H10O5)n, a polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→ 4) linked D glucose units.

Hence option 1 is the answer.

Practice Problems

1. Glucose gives silver mirror with ammoniacal silver nitrate because it has

1) aldehyde group

2) ester group

3) ketone group

4) alcoholic silver nitrate

2. Lactose gives on hydrolysis

1) glucose

2) glucose and galactose

3) fructose

4) glucose and fructose

3. The two forms of D-glucopyranose obtained from the solution of D-glucose are called

1) isomers

2) anomers

3) epimers

4) enantiomers

4. Water insoluble component of starch is

1) amylopectin

2) amylose

3) cellulose

4) None of these

5. Which of the following enzymes hydrolysis starch to glucose?

1) Amylase

2) Invertase

3) Lactase

4) Maltase

Frequently Asked Questions

What are monosaccharides?

The simplest carbohydrates which cannot be hydrolysed to smaller molecules. Their general formula is (CH2O)n where n = 3 – 7
Example : glucose, fructose etc.

Why cannot vitamin C be stored in our body?

Vitamin C is not stored in our body because it is a water-soluble vitamin. It gets excreted from the body through sweat or urine. So they must be supplied in the diet regularly.

What are the expected products of hydrolysis of lactose?

On hydrolysis, lactose gives β-D-galactose and β-D-glucose.

What is a glycosidic linkage?

The two monosaccharide units are joined together through an oxide linkage formed by loss of a molecule of water. Such a linkage between two monosaccharide units through an oxygen atom is called glycosidic linkage.

Where does the water present in the egg go after boiling the egg?

A process that changes the physical and biological properties of proteins without affecting the chemical composition of protein is called denaturation of proteins. In an egg, denaturation of protein is the coagulation of albumin present in the white of an egg. When an egg is boiled, the globular proteins (albumin of egg white) coagulate (denature) to a rubber-like insoluble mass. This insoluble mass absorbs all water present in the egg as it makes hydrogen bonds with it.

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