Molecular Biology MCQ

Molecular biology is the study of Biology at molecular level. It is mainly concerned with the interrelationships between DNA, RNA and protein synthesis. A molecular biologist studies the processes of replication, translation and transcription of genetic material on a wide scale.

Given below are molecular biology mcqs for the better understanding of concepts related to molecular biology. The answers are also provided below for reference.

Molecular Biology MCQs

1. The function of β subunit of polymerase is

  1. Template binding
  2. Catalytic binding
  3. Promoter binding
  4. Cation binding

2. Which of the σ factors is heat stable?

  1. σ 54
  2. σ 70
  3. σ 28
  4. σ 32

Also Read: DNA replication

3. The mRNA codon of valine is

  1. GUC
  2. UGG
  3. CCA
  4. TTG

4. True replication of DNA is due to

  1. Phosphate backbone
  2. Hydrogen bonding
  3. Complementary base pairing rule
  4. None

5. Which of the following statements is not applicable to viruses?

  1. The virus replicates in a bacterial host
  2. The protein coat of a virus does not enter the host cell
  3. The genetic material is DNA or RNA
  4. Virus replicate autonomously in the absence of host

6. Mode of DNA replication is

  1. Conservative and bidirectional
  2. Semiconservative and unidirectional
  3. Semiconservative and bidirectional
  4. Conservative and unidirectional

Also Read: Transcription

7. Which enzyme is used to join nicks in the DNA strand?

  1. Primase
  2. DNA polymerase
  3. DNA ligase
  4. Endonuclease

8. Which enzyme is used in the unwinding of DNA?

  1. Ligase
  2. Topoisomerase
  3. Helicase
  4. Exonuclease

9. Which of the following processes does not occur in prokaryotes?

  1. Transcription
  2. Splicing
  3. Translation
  4. Replication

10. cDNA is synthesised from RNA by the enzyme

  1. DNA polymerase
  2. DNA synthetase
  3. DNA convertase
  4. Reverse transcriptase

11. Which of the following base-pairing rule is correct?

  1. Adenine with guanine and thymine with cytosine
  2. DNA base pairing is non-specific
  3. Adenine with cytosine and guanine with thymine
  4. Adenine with thymine and guanine with cytosine

Also Read: Translation

12. DNA synthesis can be measured by estimating the incorporation of radiolabelled

  1. Thymine
  2. Guanine
  3. Cytosine
  4. Adenine

13. How many RNA polymerases are present in a bacterial system?

  1. 4
  2. 2
  3. 1
  4. 3

14. During DNA replication the synthesis of the leading strand of DNA results in fragments known as

  1. Okazaki fragments
  2. Satellite segments
  3. Kornberg segment
  4. Double-helix segment

15. Short strands of ——- primer are used in DNA replication.

  1. DNA
  2. RNA
  3. Histone
  4. Protein

Also Read: DNA Structure

Answer Key

1- b 2- d 3- a 4- c 5- d
6- c 7- c 8- c 9- b 10- d
11- d 12- a 13- c 14- a 15- b

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