Antibiotic resistance genes are used as selectable markers in E. coli as it does not contain antibiotic resistance genes naturally. Further... View Article
Selectable markers help in eliminating non-transformants and selectively grow transformants. Further reading: Plasmid What Is EcoR1? MCQs... View Article
pBR322 contains two selectable markers, i.e. antibiotic resistance genes for ampicillin (ampR) and tetracycline (tetR). Further reading:... View Article
Selectable markers help in identifying and selecting non-transformants and transformants, e.g. antibiotic resistance genes. They are used in... View Article
Selectable markers help in selecting transformants and non-transformants. E.g. antibiotic resistance genes. Further reading: Plasmid What... View Article
Restriction modification system is present in bacteria, which provides defence against foreign DNA. Restriction endonuclease cuts the DNA... View Article
Hamilton O. Smith discovered and isolated the first site-specific restriction endonuclease HindII from the bacterium Haemophilus influenzae.... View Article
The two main types of endonuclease are: DNase, which is relatively non-specific to the sequence. Restriction endonuclease or restriction... View Article
It is important that the pieces of DNA are cut with the same restriction enzymes to produce complementary sticky ends for ligation. Further... View Article
Restriction endonuclease cuts a DNA at a specific location within the DNA so it is called restriction endonuclease. Further reading: Plasmid... View Article
Type 2 restriction enzymes are used for cloning because they generally cut the DNA at a specific position within the recognition site. Further... View Article
The first type 2 restriction endonuclease discovered was HindII. It was isolated from the bacterium Haemophilus influenzae. Further reading:... View Article
Restriction endonuclease present in the bacteria cleaves the foreign DNA and protects it from infection. It cuts the DNA at specific sequences... View Article
Gene cloning involves the insertion of a gene of interest to a cloning vector to form recombinant DNA and then transportation of the foreign DNA... View Article
Cloning is used to produce proteins, vaccines, and antibiotics. Cloning in agriculture is used to produce pest-resistant plants. Cloning is also... View Article
The minimum features required in a cloning vector are the origin of replication, a selectable marker and cloning sites. Further reading:... View Article
