RNA polymerase is located in the nucleus and also in mitochondria and chloroplast. Further reading: DNA Polymerase Polygenic Inheritance... View Article
DNA polymerase is used in PCR to synthesise and amplify new DNA strands. Further reading: DNA Polymerase Polygenic Inheritance Chromosome... View Article
DNA polymerase III is the main replication enzyme, which catalyses the polymerisation reaction. Other DNA polymerases are involved mainly in... View Article
DNA Polymerases are a group of enzymes that catalyse the synthesis of DNA during replication. The main types of DNA polymerase in prokaryotes are... View Article
DNA polymerase adds complementary nucleotides at the 3’ OH group of the growing strand and catalyses the replication in the 5’ to 3’ direction.... View Article
Yes, DNA polymerase requires a primer as it can only add nucleotides to the growing polypeptide chain. It cannot initiate replication. Further... View Article
DNA polymerase catalyses the replication of DNA and produces new DNA strands, whereas, RNA polymerase catalyses transcription, i.e. synthesis of... View Article
The main function of DNA polymerase I is to repair DNA and remove RNA primer from Okazaki fragments. It has both 5’→3’ and 3’→5’ exonuclease... View Article
The three different roles played by DNA polymerase are polymerisation, repair and proofreading. They show polymerisation activity in the 5’→3’... View Article
Ligase catalyses the joining of two molecules forming a bond, whereas lyase catalyses the breaking of a bond by double bond formation, i.e.... View Article
DNA ligase joins the discontinuously synthesised Okazaki fragments during replication. Further reading: Polygenic Inheritance Chromosome... View Article
DNA ligase requires ATP to join together the two DNA strands. Further reading: Polygenic Inheritance Chromosome Structure Molecular Basis... View Article
Okazaki fragments are short DNA fragments, which are synthesized discontinuously to form the lagging strand during replication. They are joined... View Article
DNA ligase catalyses the formation of a phosphodiester bond between the 3’ hydroxyl group of one nucleotide to the 5’ phosphate group of another... View Article
DNA polymerase adds nucleotides to the growing DNA strand, whereas DNA ligase joins the breaks in DNA having matching ends during replication,... View Article
