To prepare a temporary stained mount of a transverse section of dicot and monocot stem and root to study various plant tissues.
- Preserved material of sunflower root and stem
- Preserved or fresh material of maize root and stem
- Sharp blade
- Watch glass
- Safranin (1gm in 100ml of 50% ethanol)
- Blotting paper
T.S Of Dicot Stem
T.S Of Monocot Stem
- Hold the plant material that is dissected between your index finger and thumb while keeping the edge of the razor perpendicular to the longitudinal axis of the plant. Slice it into thin sections
- Using the edge of the blade, shift these sections from the blade’s edge into a watch glass with the aid of a brush. The watch glass must hold water
Process Of Staining
- Pick 2 to 4 thin and good transverse sections. Shift it to a different watch glass holding safranin stain
- Let the sections rest in the stain for a couple of minutes
- Drain the sections of the stain with water so as to wash off the excess strain
- On a clean slide, place a stained section in the middle of the slide, mounting water or glycerine.
- Using a needle slowly place the coverslip
- Excess of water or glycerine from the edge of the coverslip can be removed using blotting paper
- Ensure air bubbles are not formed while mounting
- While dissecting the section, both the blade and the material should be supplied with adequate water
- While working with sections, use a brush
- Gentle placement of coverslip in order to avoid air bubbles
- Excess glycerine can be removed with filter paper
To study the transverse section of a dicot stem, a sunflower stem.
- A typical example of a dicot stem is a sunflower
- The outline of the stem in the transverse section is circular with a hairy surface
- The transverse section depicts the arrangement of different tissues in a particular way from the exterior to the center as listed below:
- It is the outermost covering of the stem and is single layered
- The cells are living cells that are densely arranged having a thin wall, enclosed in a cuticle
- It displays multicellular hair
- Function – It provides protection
- Located just under the epidermis
- It consists of the following regions – Hypodermis, Endodermis, General cortex
(a) Hypodermis – It is situated just below the epidermis and consists of 4 to 5 layers of collenchymatous cells. These are cells that have the cellulose deposits at the edges and are living cells. It may possess chloroplast and hence carry out photosynthesis. Function: It renders mechanical support
(b) General Cortex (Parenchyma) – Located just under the hypodermis, it consists of several layers of cells that are living having thin walls along with intercellular spaces. It may contain chloroplast and hence become photosynthetic. Several mucilaginous canals can also be observed. Function: Food storage
(c) Endodermis – Forms the interior most covering of the cortex consisting of a singular row of cells that are densely arranged and barrel-shaped with no intercellular spaces. They are composed of starch grains.
In tissue, it forms the central core having a vascular bundle. It comprises of – pericycle, vascular bundles, medullary rays, and pith.
- Located between the vascular bundles and endodermis
- Has recurrent patches of parenchyma and sclerenchyma
- Each schlerenchyma patch is linked with hard-blast fibers
(b) Medullary rays
- It is located in gaps between the vascular bundles. This gap finds parenchymatous cells that are thinly walled and arranged radially in 4-5 rows.
- Helps in the storage of food material and in the lateral conduction of water and food
(c) Vascular bundle
- They are open, collateral and conjoint and are set up in a ring-like manner
- Each of the vascular bundle comprises of- xylem, phloem, cambium
- Phloem is located below the pericycle towards the exterior side. It consists of thin cell walls possessing companion cells, sieve tube and phloem parenchyma and conducts food material
- Xylem is found in the interior of the vascular bundle, i.e., smaller protoxylem towards the center and larger metaxylem towards the periphery. Therefore xylem is endarch. These cells are dead and lignified. Xylem conducts minerals and water
- Cambium is present between phloem and xylem. The cells are rectangular and have thin cell walls. It consists of meristematic tissues that generate new cells
- It forms the mid-region of the stem. It arises under the vascular bundle up till the center and consists of large parenchymatous cells.
- The epidermis has multicellular hairs
- The hypodermis is collenchymatous
- The xylem is endarch with the protoxylem being at the center and the metaxylem being at the periphery
- Vascular bundles are open, collateral and conjoint
- They are arranged in a ring-like manner
- Pith is found at the center
The specimen provided is a section of a dicot stem.
To study the transverse section of the dicot root, sunflower root.
The T.S shows the following structures:
- Epiblema or Epidermis
- Densely arranged cells with thin cell walls forming the outermost single layer
- Several cells have thin outgrowths known as root hairs
- Present under the epidermis consisting of several layers of thin-walled parenchyma cells
- They are barrel-shaped forming the innermost layer of the cortex. They are densely arranged single cell layer.
- A Casparian band is formed due to the radially arranged cell walls that are thickened
- Under the endodermis is a thin-walled layer of cells known as pericycle.
- It consists of radial bundles having alternating 2 to 6 arrangement of bundles of phloem and xylem
- Bundles of xylem are exarch wherein the metaxylem is present towards the center and the protoxylem at the outer side
- In outline, xylem vessels are polygonal
- Comprises of companion cells, parenchyma and sieve tube
- They are the parenchyma cells that separate the xylem and phloem bundles from each other
- They are significantly absent or occur rarely
- The epidermis shows the presence of unicellular hair
- Absence of hypodermis
- Radially arranged vascular bundles
- The number of phloem/xylem bundles is not more than 6
- The xylem is exarch
- Absence or rare occurrence of pith
The specimen provided is a section of the dicot root.
To study the transverse section of monocot stem, maize stem.
- The maize plant is a monocot plant
- The transverse section is circular having a smooth surface
- The ground tissue consists of many scattered vascular bundles
- From the periphery to the center, the transverse section displays the structure of different tissues in a particular manner as follows:
- An outermost, single layer
- Thin-walled cells that are living. Presence of thick cuticle towards the outermost surface
- The rare occurrence of stomata.
- Lack of epidermal hair
- Function – Protection of internal tissue
- Present under the epidermis
- Consists of the thick-walled dead layer of sclerenchymatous cells. Made up of two of these three layers
- Found below the hypodermis
- Consists of thin-walled living cells, parenchymatous cells
- Loose arrangement of cells with the presence of intercellular spaces
- As seen in the dicotyledonous stem, there is no differentiation of cortex, pericycle and the endodermis
- Several scattered vascular bundles found in the ground tissue and are closed type, collateral and conjoint
- At the periphery, the vascular bundles are more and densely arranged when compared to the center
- Large vascular bundles in the center compared to vascular bundles at the periphery
- They resemble an oval shape that is girdled by sclerenchymatous bundle sheath
- It consists of the xylem and the phloem
- Metaxylem are present at the two lateral arms. Protoxylem is present at the base
- The lower protoxylem particles form a cavity known as lysogenous cavity
- Function: Conducts water
- Present at the periphery and consists of living cells
- It comprises of companion cells, sieve tubes, and the phloem parenchyma
- Function – Conducts food material
The specimen provided is a section of the monocot stem.
To study of the transverse section of monocot root, maize root.
The transverse section of monocot root depicts the structures as listed below:
- Epiblema or Epidermis
- It is a single outermost layer with no cuticle
- Densely arranged cells
- Few cells may see unicellular root hair emerging
- Found below epidermis
- It is broad consisting of multiple layers of parenchyma cells
- The innermost layer of the cortex
- Barrel-shaped cells arranged in a ring-like manner
- Presence of Casparian bands
- Found below the endodermis and is a single layer of parenchymatous cells
- There are 8 or more alternating bundles of phloem and xylem known as radial bundles
- Xylem bundles are exarch
- Xylem bundles are oval or rounded
- Phloem is found under the pericycle and comprises of companion cells, sieve tube, parenchyma
- It is the xylem and phloem bundles that are distinguished from each other by the parenchyma tissue
- Well developed pith is observed
- It comprises of the parenchyma in the mid-region of the root
- Epidermis sees the presence of unicellular hair
- Absence of hypodermis
- Radially arranged vascular bundles
- 8 or more than 8 phloem and xylem bundles
- A well-developed pith
The specimen provided is a section of the monocot root.
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