Nucleases degrade the molecules of DNA by disintegrating the phosphodiester bonds which associate one nucleotide with the adjacent one in the... View Article
The main aim of amplification is to increase the copy number of a plasmid. Some multicopy plasmids have the beneficial attribute of being able to... View Article
Bacterial cell extract has good quantities of RNA and protein. Several methods can be used to purify DNA from such a mixture. Treating the... View Article
The process for the total DNA preparation from a culture of a bacterial cell can be categorized into four stages - Culture of bacteria is grown... View Article
Often, the complementary single strands are referred to as cohesive ends or 'sticky' ends. This is because the base pairing between them can... View Article
As opposed to the lytic cycle, lysogenic infections are characterized by the retention of the phage DNA molecules in the host bacterium,... View Article
The typical infection pattern (same for all phage types) is a three-step process: Phage substances bind to the exterior of the bacterium,... View Article
Plasmids, based on the prime characteristic coded by the plasmid genes, can be classified into the following types: R or resistance plasmids -... View Article
Plasmids can be categorized as conjugative and nonconjugative. These conjugative plasmids are distinguished by their ability to facilitate sexual... View Article
Copy number and size of a plasmid are particularly important as far as cloning is concerned. Copy number is the number of molecules of an... View Article
Congenital disorders (birth defects) can be classified as follows - Classification Example Malformation or congenital abnormality Congenital... View Article
The phenotype of an entity is a result of two fundamental factors - the genotype or the expression of the genetic code of an entity and the... View Article
Inheritance of chromosomes occurs through meiosis. Every time there is a cell division, the 23 pairs of chromosomes (for humans) in cells are... View Article
Secretion of hormones is influenced by many factors such as - Neurotransmitters or hypothalamic peptides (Stimulatory and Inhibitory agents)... View Article
The negative-feedback mechanism (Short-Loop, long-loop negative Feedback) controls the regulation of secretion of a few hormones from the... View Article
Some of the characteristics of Hydrophilic hormones receptors are - Association between receptor and hormone is reversible For a particular... View Article