Apart from all the limitations PCR poses, it does have its share of advantages too. The polymerase chain reaction is a simple method to use and... View Article
An ideal example of PCR is the analysis of tumours. It could be used to isolate and the amplification of DNA of the tumour suppressor genes or... View Article
When polymerase chain reaction (PCR) is normally utilized to find the absence of presence of a particular DNA product, it is referred to as a... View Article
The main two methods to visualize PCR products - One is staining of amplified DNA product with ethidium bromide (chemical dye) intercalating... View Article
The PCR or polymerase chain reaction is a sensitive procedure that enables quick amplification of particular fragments of DNA. The polymerase... View Article
Limitations of the polymerase chain reaction are - Specificity of the PCR product generated can be changed by the non-specific binding of... View Article
Each PCR assay necessitates the presence of template DNA, nucleotides, primers and DNA polymerase. The DNA polymerase is the key enzyme that... View Article
It is the most significant piece of technology for scientists who want to make use of polymerase chain reaction (PCR). Thermocycler tightly... View Article
In this step, the reaction's temperature is increased to the feasible temperature for the polymerase. The polymerase produces new DNA which... View Article
In 1976, for the first time, the operative to polymerase chain reaction was discovered. It was the Taq polymerase which was purified from the... View Article
PCR (Polymerase chain reaction) makes use of the natural role of polymerase enzymes. In a cell that divides normally, the duplication of genes... View Article
Polymerase chain reaction (PCR) includes annealing or hybridization as the second step. The Taq polymerase needs a short fragment of RNA to start... View Article
It is a technique that is used to distinguish two main classes of bacteria on the basis of their differing constituents of the cell wall. The... View Article
From the 4th to the 6th day of the cycle, the regeneration of the endometrium is performed by the stratum basale. The epithelium of the fundi of... View Article
The two peritoneal ligaments that are attached to the ovary are - Ligament of the ovary - it extends from the ovary to the fundus of the uterus,... View Article
The three significant histological features of the ovary are - Cortex - comprises connective tissue stroma and many ovarian follicles. Each of... View Article
The dominant egg in post-pubertal females continues developing, depending on the hormones for stimulation and growth. Upon the full maturation of... View Article
Many primordial follicles are triggered to continue the development however, only one follicle completes the development to liberate the ovum.... View Article
Once the ovum is released, the remaining cells of the granulosa and theca interna lead to the formation of the corpus luteum. The centre... View Article
The salient feature that differentiates secondary from primary follicles is the appearance of the follicular antrum in the granulosa layer. The... View Article
