India UK Migration Pact:- Download PDF Here Anchor: Teena Jha Participants:Â Gurjit Singh, Former Ambassador. Prof. Harsh V. Pant, Head,... View Article
UPSC Current Affairs Preparation: The Hindu Analysis Watch expert analysis of ‘The Hindu’ dated 18th May 2021. Important news and views to boost... View Article
NASA and Texas-based company Axiom Space have agreed on terms for the first private astronaut mission to the International Space Station, which... View Article
The first India Energy Outlook report was released in 2015 by the International Energy Agency (IEA). Six years later, the organization has... View Article
Lyrebird is an Australian Bird known for its ability to mimic artificial and natural sounds from the environment. In the past, UPSC has surprised... View Article
29th July is celebrated as the Global Tiger Day, often referred to as International Tiger Day. 13 Tiger Range Countries participated in the Saint... View Article
CNA 18th May 2021:- Download PDF Here TABLE OF CONTENTS A. GS 1 Related B. GS 2 Related POLITY AND GOVERNANCE 1. Why and how of creating a... View Article
By the incubation of enzymes with a reducing agent, the exonuclease activity is fully inactivated. Lower concentrations of ferrous ions,... View Article
It is a profile of the restriction endonucleases cleavage in a fragment of the DNA. The generation of the restriction map are the first steps in... View Article
These are different Restriction endonucleases that have the same recognition site. In a few cases, it identically cuts within its recognition... View Article
The enzyme snips the double-stranded DNA. Specifically, it splits the sugar-phosphate backbone of the DNA without any damage to the bases. The... View Article
It is a fluorescent dye intercalating between the bases of nucleic acids. It permits very convenient and easier identification of the DNA... View Article
Many buffers are suggested for the same, the most common one for duplex DNA is Tris-acetate EDTA (TAE) and Tris-borate-EDTA (TBE). The DNA... View Article
Bacteriophages replicate through the lytic phase cycle while the phage genome is inserted into the cell. The phage genes are expressed and the... View Article
It is used in comparing gene expressions under different conditions. For instance, a scientist can compare genes expressed by E.coli at lower... View Article
It is a screening method allowing scientists to determine genes that are missing. For instance, the genes accountable for a hereditary defect can... View Article
It is the tightly coiled promoter region that is deemed to be the most significant feature. Proteins of the expression library are only expressed... View Article
It is a mutant version of the lac promoter, the lacUV promoter drives a high level of transcription, in induced conditions only. Its components... View Article
As foreign particles, particularly if synthesized in larger quantities, could be toxic to E.coli, the promoter utilized to express the foreign... View Article
In such libraries, vectors have sequences necessary for translation and transcription of inserts. This indicates that insert DNA is expressed as... View Article
