Normal PCR uses DNA as a template, whereas in RT-PCR (reverse transcription PCR), RNA is used as a template. Further reading: DNA Polymerase... View Article
A specific part of the viral genome is amplified using a primer and can be detected even at a low concentration if present in the sample.... View Article
Primers are the short stretch of nucleotides, i.e. oligonucleotides, which are complementary to the DNA strand to be replicated. DNA polymerase... View Article
PCR can be used in the early detection of a disease when the concentration of pathogen is less in the body, e.g. HIV, malaria, anthrax,... View Article
We need primers in PCR because DNA polymerase can only add nucleotides to the growing strand. Further reading: DNA Polymerase RNA Splicing... View Article
In the real-time PCR, the accumulation of the amplified product is quantified after each cycle as the reaction progresses. Further reading:... View Article
The main function of polymerase chain reaction is to amplify and make multiple copies of a piece of DNA of interest. Further reading: DNA... View Article
Polymerase chain reaction requires a DNA template, primers and thermostable DNA polymerase. Further reading: DNA Polymerase RNA Splicing... View Article
Polymerase chain reaction is a technique to amplify a piece of DNA or a gene. Multiple copies of the DNA sample are prepared by using two primers... View Article
Polymerase chain reaction is a technique to amplify or make multiple copies of a DNA sample. It is widely used in recombinant DNA technology,... View Article
DNA mapping refers to the methods which tell about the position of genes on chromosomes and the distance between them. Further reading: DNA... View Article
The crossover frequency is proportional to the distance between the two genes, i.e. greater the distance between two genes, the greater is the... View Article
Vertical gene transfer refers to the transfer of genetic material from the mother to the daughter cells during cell division. Further reading:... View Article
During the transformation in Pneumococcus, the R-strain bacteria had been transformed and became virulent by the heat-killed S-strain bacteria.... View Article
