Type 2 restriction enzymes are used for cloning because they generally cut the DNA at a specific position within the recognition site. Further... View Article
The first type 2 restriction endonuclease discovered was HindII. It was isolated from the bacterium Haemophilus influenzae. Further reading:... View Article
Restriction endonuclease present in the bacteria cleaves the foreign DNA and protects it from infection. It cuts the DNA at specific sequences... View Article
Gene cloning involves the insertion of a gene of interest to a cloning vector to form recombinant DNA and then transportation of the foreign DNA... View Article
Cloning is used to produce proteins, vaccines, and antibiotics. Cloning in agriculture is used to produce pest-resistant plants. Cloning is also... View Article
The minimum features required in a cloning vector are the origin of replication, a selectable marker and cloning sites. Further reading:... View Article
The foreign DNA is inserted into a plasmid by cutting the DNA and plasmid by the same restriction enzyme and then ligating the foreign DNA into... View Article
Yes, pBR322 is an artificial cloning vector created in the laboratory of Herbert Boyer. BR stands for the names of the scientists, Bolivar and... View Article
pBR322 is a good cloning vector because it contains two antibiotic resistance genes that act as a selectable marker and recognition sites for... View Article
DNA vectors are used for cloning. They are used for transferring foreign DNA into another cell. Further reading: Plasmid What Is EcoR1?... View Article
Yeast artificial chromosome (YAC) is used for cloning larger DNA fragments. It can be used to clone up to 3000 kb of DNA fragment. Further... View Article
Yes, plasmids are small, circular and double-stranded DNA that replicate autonomously. They are extrachromosomal DNA molecules. Further reading:... View Article
Plasmids are used as cloning vectors in genetic engineering because they can replicate independently and can transfer the desired gene to the... View Article
Plasmids are self-replicating extrachromosomal DNA present in bacteria. They contain cloning sites where foreign DNA can be inserted, a... View Article
The main characteristics of a cloning vector are: Origin of replication for replication within the host cell. Selectable markers such as... View Article
Both plasmid and foreign DNA are cut by the same restriction enzyme and then the foreign DNA is ligated into the complementary site of the... View Article
