pBR322 is a plasmid. It contains various restriction sites in the antibiotic resistance genes, tetracycline and ampicillin. It is used for... View Article
The main types of cloning vectors are plasmids, bacteriophages, cosmids, yeast artificial chromosomes (YACs), bacterial artificial chromosomes... View Article
Cloning vectors are a small genome, where foreign DNA can be inserted and delivered to the host cell that can be used for cloning purposes. E.g.... View Article
Cloning vectors are used to insert foreign DNA into a host cell and make multiple copies of the inserted DNA. Further reading: Plasmid What... View Article
Sticky ends or overhanging single-stranded stretches cut facilitates ligation by DNA ligase due to the formation of hydrogen bonds between... View Article
Restriction enzymes break phosphodiester bonds in the sugar-phosphate backbone of the DNA strands. Further reading: Plasmid What Is EcoR1?... View Article
The type II restriction enzyme is commonly used in recombinant DNA technology. Further reading: Plasmid What Is EcoR1? MCQs On Lac Operon... View Article
Type II restriction endonucleases cut DNA at a specific site, mostly within the recognition site, hence they are used for cloning. Further... View Article
Restriction enzymes recognise the specific base sequences called recognition sequence that determines the cleavage site. Further reading:... View Article
