Method - Desired DNA or gene, with the help of restriction enzymes, are isolated Both plasmid and desired gene are treated with the same... View Article
Insertion of the plasmid into the cell again - in this step, the circular plasmid DNA which now comprises the host gene is introduced into the... View Article
Genetic engineering includes several steps, two of which are - Isolation of genes - genes to produce a protein is isolated from cells. Then the... View Article
A variation in one of the PCR techniques can be used in the determination of evolutionary relationships between organisms. The PCR product could... View Article
It is a method used in the quick assessment of the measure of PCR product (real-time preferably). As a result, it is an indirect procedure for... View Article
Reverse Transcription PCR (RT-PCR) is a procedure made for identification, isolation and amplification of a known sequence forming a cell or... View Article
Touchdown PCR is a variant of PCR that decreases the nonspecific primer annealing. This is done by lowering the annealing temperature between... View Article
It is used in the detection of methylation of the CpG islands in the genomic DNA. Here, DNA is treated first with sodium bisulfite that then... View Article
Inter Sequence-specific PCR or ISSR is a method of PCR used for the DNA fingerprinting, which amplifies the sites between the simple sequence... View Article
It is a method that decreases the non-specific amplification in the initial establishment phase of the PCR. It can be manually conducted by... View Article
It is utilized to amplify preferentially 1 strand of the actual DNA more compared to its counterpart. This type finds applications in a few kinds... View Article
The process of detecting hereditary diseases provided a genome is a tedious and long, challenging process that could be remarkably shortened with... View Article
It is a method used to permit PCR only when one internal sequence is cognized or known. This is particularly used in the identification of... View Article
The process of PCR can be split into three stages - Exponential amplification - at every cycle, the amount of product gets doubled. The reaction... View Article
The DNA fragments which are to be amplified are determined by selecting primers. Two primers are used, which determine the beginning and end... View Article
Polymerase chain reaction (PCR) needs many fundamental components, which are as follows - The cDNA or DNA template comprising the regions of DNA... View Article
Polymerase chain reaction is utilized in the amplification of a well-defined, short part of a DNA strand. It can be a single gene or even a... View Article
PCR is a widely used, simple process wherein small amounts of DNA could be amplified in several copies. Along with quickly functioning, it can... View Article
