As foreign particles, particularly if synthesized in larger quantities, could be toxic to E.coli, the promoter utilized to express the foreign... View Article
In such libraries, vectors have sequences necessary for translation and transcription of inserts. This indicates that insert DNA is expressed as... View Article
If a researcher is trying to locate a specific gene in the target entity, the probe for the library can be the corresponding gene from the... View Article
Screening DNA libraries by the process of hybridization necessitates the preparation of the library DNA and preparation of the labelled probe. A... View Article
It requires the preparation of the DNA library and preparing the labelled probe. Gene libraries are stored as a bacterial culture of E.coli cells... View Article
Gene libraries are used in finding new genes for sequencing the complete genome, comparing genes from different entities and other purposes too... View Article
They hold the large fragments of DNA including yeast artificial chromosomes, P1 bacteriophage artificial chromosomes, bacterial artificial... View Article
Even though several special vectors exist now, these are some attributes that are convenient and seen in most of the modern generalized cloning... View Article
Cloning vectors are special plasmids holding a fragment of foreign DNA to study or manipulate further. The types and numbers of plasmids that are... View Article
Dot blots start with spotting RNA or DNA samples onto the nylon membrane. Usually, various concentrations of the sample are dotted in a side by... View Article
Northern blots are based on nucleic acid hybridization, the difference being that RNA is the target in the northern blot. For the northern blot,... View Article
It is used to find how closely related DNA is from one source compared to a sequence of DNA from the other. This technique uses forming hybrid... View Article
If two differing double helixes of DNA melt, before reannealing and cooling, the single strands can be mixed together. If two original molecules... View Article
Biotin is a vitamin, digoxigenin is a steroid from the plant of foxglove. With the help of these chemicals, scientists can label DNA without any... View Article
They can be identified with the help of scintillation counting where small samples of the radioactive DNA are blended with the scintillation... View Article
The recombinant DNA methodologies necessitate the capability to detect DNA. One of the ways to find the amount of RNA or DNA in a solution is by... View Article
All the fragments tend to have identical overhangs when two varying samples of DNA are snipped with a similar sticky-end restriction enzyme. This... View Article
The key to making DNA fragments is restriction enzymes that naturally occur or the restriction endonucleases. Such bacterial enzymes associate... View Article
The type of gel used is affected by the size of the DNA which is examined. The standard one is agarose, however, for tiny DNA fragments ranging... View Article
Once sequencing reactions take place, the mixture of strands of varying lengths and all endings of ddNTP (fluorescently labelled) has been... View Article
