PCR cloning is used to make multiple copies of a DNA piece and make recombinant DNA molecules. It is used in gene cloning, gene quantification... View Article
The restriction enzyme HindIII produces sticky ends. The recognition site is a palindrome having the sequence AAGCTT. It cuts the DNA between the... View Article
HaeIII leaves blunt ends as it cuts both the DNA strands at the same location. Further reading: Plasmid What Is EcoR1? MCQs On Lac Operon... View Article
EcoRI leaves sticky ends with AATT overhangs. Further reading: Plasmid What Is EcoR1? MCQs On Lac Operon Genetically Engineered Insulin... View Article
Sticky ends have single strand overhangs, blunt ends do not have single strand overhangs, it terminates in a base pair. Further reading:... View Article
Sticky ends are better than blunt ends because they facilitate ligation by DNA ligase by forming hydrogen bonds between complementary bases of... View Article
DNA fragments cut by restriction enzymes are called restriction fragments. They can be separated by gel electrophoresis. Further reading:... View Article
Restriction enzymes are known as molecular scissors. They are endonucleases and cut the DNA at specific points. Further reading: Plasmid... View Article
Restriction sites have a specific 4-8 nucleotide long sequence which is recognised by the restriction enzymes. Restriction enzymes cut DNA within... View Article
The recognition site for EcoRI is a palindrome. It is a six nucleotide sequence containing GAATTC in 5’ to 3’ direction and the complementary... View Article
Recognition site is a 4-8 nucleotide long sequence present on DNA, which is recognised by the restriction enzyme. Further reading: Plasmid... View Article
Recognition site is the sequence of bases that are identified by restriction enzymes for cutting DNA at a specific site. Further reading:... View Article
DNA is negatively charged so it moves towards the anode. The DNA fragments separate according to their size. The smallest fragment moves the... View Article
pUC plasmid is a cloning vector created by modifying pBR322. UC stands for the University of California, where it was synthesised. It is smaller... View Article
pUC18 is a plasmid cloning vector. It was synthesised at the University of California. Yanisch-Perron et al created pUC18. Further reading:... View Article
pUC18 is a plasmid cloning vector. It consists of 2686 bp. It is commonly used in E. coli. Further reading: Plasmid What Is EcoR1? MCQs... View Article
When foreign DNA is inserted into the gene coding for an enzyme, it results in the inactivation of the gene, which synthesises the enzyme. It is... View Article
