The term plasmid was coined by J Lederberg in 1952 to describe extrachromosomal genetic material. Further reading: Plasmid What Is EcoR1?... View Article
The first cloning vector was produced by Herbert Boyer and Stanley Cohen. It was a plasmid vector called pSC101. Further reading: Plasmid... View Article
pBR322 is a useful vector because it contains antibiotic resistance genes as a selectable marker and recognition sites for the action of... View Article
Reporter gene is a gene that is attached to the regulatory sequence to be studied. It imparts a specific characteristic, which can easily be... View Article
Cloning site is the site where DNA is inserted into the vector. Restriction enzyme recognises the recognition site and cuts the DNA for insertion... View Article
Ori site is responsible for initiating the replication and the cloning site is where the foreign DNA is inserted into the vector. Further... View Article
Ori site is required for initiation of replication, if the ori site is absent then replication will not start. Further reading: Plasmid... View Article
Transformants are the cells that have taken up the foreign DNA by transformation, whereas non-transformants are the cells that have not undergone... View Article
Selectable markers are the genes present in the cloning vectors that help in eliminating the non-transformants. E.g. antibiotics resistance... View Article
Cloning plasmids contain selectable markers so that the plasmid containing the DNA insert can be separated from the non-recombinants ones.... View Article
The gene z codes for the enzyme -galactosidase. It is responsible for the hydrolysis of lactose, a disaccharide to two monosaccharides, i.e.... View Article
