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Question
What is pUC18?
What is pUC18?
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Solution
pUC18:
- The plasmid cloning vector pUC18 is widely used in E.coli.
- A plasmid is a circular dsDNA molecule with a circumference of a few hundred or thousand base pairs. Plasmids are bacterium viruses that exist naturally.
- Because of its modest size, pUC18 allows big DNA fragments to be successfully cloned.
- In appropriate host organisms, the plasmid pUC18 transmits ampicillin resistance and complement deficiencies in beta-galactosidase.
- The MCS region of pUC18 is inverted compared to pUC19. Both pUC18 and pUC19 allow you to clone restriction enzyme-generated DNA fragments in both directions towards the lac promoter.
pUC18:
- The plasmid cloning vector pUC18 is widely used in E.coli.
- A plasmid is a circular dsDNA molecule with a circumference of a few hundred or thousand base pairs. Plasmids are bacterium viruses that exist naturally.
- Because of its modest size, pUC18 allows big DNA fragments to be successfully cloned.
- In appropriate host organisms, the plasmid pUC18 transmits ampicillin resistance and complement deficiencies in beta-galactosidase.
- The MCS region of pUC18 is inverted compared to pUC19. Both pUC18 and pUC19 allow you to clone restriction enzyme-generated DNA fragments in both directions towards the lac promoter.
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