Question

What is pBR322 used for?

Answer 1

pBR322:

1. pBR322 is a plasmid and was one of the first widely used E.coli cloning vectors.
2. It was developed by Boliver and Rodriguez, hence, BR is used in the nomenclature.
3. It has a small size of 4,363 base pairs.
4. It is isolated from E. coli.
5. It has two marker genes i.e., ampicillin and tetracycline resistant genes.
6. It is used as a tool to clone, transfer, and manipulate genes.