Question

Why is pBR322 a useful vector?

Answer 1

pBR322:

1. A vector is a DNA molecule (typically a plasmid or a virus) that is used in cloning or recombinant DNA techniques to transport a specific DNA segment into a host cell.
2. Plasmids are vectors that are extensively used in recombinant DNA technology.
3. pBR322 is a useful vector since it comprises antibiotic resistance genes as a selectable marker as well as recognition sites for the activity of restriction enzymes.
4. pBR322 is a plasmid that was one of the first commonly used E.coli cloning vectors.
5. It is a 1977 modified bacterial plasmid.
6. It possesses a tetracycline and ampicillin antibiotic resistance gene, which permits the bacteria to grow in antibiotic-containing media.