Question

pBR322 vector was produced by

• A. Bolivar.
• B. Rodriguez.
• C. Milstein and Kohler.
• D. Both A and B.

Answer 1

Answer: (D)

Both A and B.

Simplest cloning vectors based on small bacterial plasmids are the most widespread in gene cloning experiment. One of the most popular vector used in gene cloning is pBR322, and its nomenclature is pBR322 plasmid, p- A plasmid, BR. The laboratory in which the vector was originally constructed (Bolivar and Rodriguez, the two researchers who developed pBR322), 322- this number distinguishes the plasmid from other plasmids developed in the same laboratory.

Therefore, the correct answer is option D.