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Question
Write the role of 'Ori' and 'restriction' site in a cloning vector pBR322.
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Solution
Ori - It is a genetic sequence that acts as the initiation site or the origin site for the replication of DNA. Any fragment of DNA, when linked to the ori region, can be initiated to replicate.Restriction site - It is the recognition site for restriction enzymes (such as EcoRI, Hind III, PvuI, BamHI, etc.). Recognition sites are the genetic sequences where the restriction enzymes cut the DNA segments.
Ori - It is a genetic sequence that acts as the initiation site or the origin site for the replication of DNA. Any fragment of DNA, when linked to the ori region, can be initiated to replicate.
Restriction site - It is the recognition site for restriction enzymes (such as EcoRI, Hind III, PvuI, BamHI, etc.). Recognition sites are the genetic sequences where the restriction enzymes cut the DNA segments.
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