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Question
How many cloning sites are there in pBR322?
How many cloning sites are there in pBR322?
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Solution
pBR322:
- pBR322 is a plasmid that is widely used in genetic engineering.
- It is one of the first and oldest used plasmids, used for cloning E. Coli.
- In the name pBR322, p stands for plasmid, B is Bolivar and R stands for Rodriguez in the name of scientists who synthesized it.
- It Contains two antibiotic resistance genes, which function as recognition sites for restriction enzymes, and selectable markers.
- pBR322 contains two selectable markers, i.e., tetracycline (tetR) and ampicillin (ampR) antibiotic resistance genes.
- The site in the plasmid pbR322 where DNA is inserted into the vector.
- Cloning sites present in pBR322 are- BamHI, HindIII, SalI, PvuI, PvuII, PstI, EcoRI, and ClaI.
Figure showing plasmid pBR322:
pBR322:
- pBR322 is a plasmid that is widely used in genetic engineering.
- It is one of the first and oldest used plasmids, used for cloning E. Coli.
- In the name pBR322, p stands for plasmid, B is Bolivar and R stands for Rodriguez in the name of scientists who synthesized it.
- It Contains two antibiotic resistance genes, which function as recognition sites for restriction enzymes, and selectable markers.
- pBR322 contains two selectable markers, i.e., tetracycline (tetR) and ampicillin (ampR) antibiotic resistance genes.
- The site in the plasmid pbR322 where DNA is inserted into the vector.
- Cloning sites present in pBR322 are- BamHI, HindIII, SalI, PvuI, PvuII, PstI, EcoRI, and ClaI.
Figure showing plasmid pBR322:
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