Ori site is responsible for initiating the replication and the cloning site is where the foreign DNA is inserted into the vector. Further... View Article
Ori site is required for initiation of replication, if the ori site is absent then replication will not start. Further reading: Plasmid... View Article
Transformants are the cells that have taken up the foreign DNA by transformation, whereas non-transformants are the cells that have not undergone... View Article
Selectable markers are the genes present in the cloning vectors that help in eliminating the non-transformants. E.g. antibiotics resistance... View Article
Cloning plasmids contain selectable markers so that the plasmid containing the DNA insert can be separated from the non-recombinants ones.... View Article
The gene z codes for the enzyme -galactosidase. It is responsible for the hydrolysis of lactose, a disaccharide to two monosaccharides, i.e.... View Article
When the recombinant DNA is inserted into the lac z gene, which codes for the enzyme -galactosidase, it gets inactivated and the recombinant... View Article
pBR322 is an artificial plasmid. 322 in pBR322 refers to the order of synthesis and helps in distinguishing it from other plasmids constructed in... View Article
ROP codes for a protein that is responsible for restricting the plasmid copy number. It is also called a repressor for primers. It is involved in... View Article
Ti plasmid of Agrobacterium tumefaciens is commonly used as a cloning vector for plants. Further reading: Plasmid What Is EcoR1? MCQs On... View Article
If there are no selectable markers in the plasmid that is chosen as a cloning vector then it will not be possible to differentiate between the... View Article
June 20th, 2021, PIB:- Download PDF Here TABLE OF CONTENTS 1. Auction of coal mines 2. Ganga Quest 2021 3. NISHTHA Capacity Building Programme... View Article
UPSC Current Affairs Preparation: The Hindu Analysis Watch expert analysis of ‘The Hindu’ dated 20th Jun 2021. Important news and views to boost... View Article
