We can know if the transformation was successful or not by the selectable marker present in the plasmid. Transformants containing antibiotic... View Article
Recombination is the process by which DNA is broken and then reunites to produce different combinations of alleles. Genetic recombination leads... View Article
Transformation is the process by which the bacterial cells take up the DNA from the surrounding environment. Further reading: Plasmid What... View Article
Transformants are selected from non-transformants by the presence of a selectable marker, e.g. transformants can be selected by plating them on... View Article
Transformants are selected by the presence of selectable markers, e.g. by growing them in an antibiotic-resistant medium according to the... View Article
Recombinants contain a piece of foreign DNA, whereas non-recombinants do not contain foreign DNA in their genome. Further reading: Plasmid... View Article
Recognition sequences are the site, which is recognised by restriction endonucleases and they cleave the DNA at or near the recognition site.... View Article
PCR cloning is used to make multiple copies of a DNA piece and make recombinant DNA molecules. It is used in gene cloning, gene quantification... View Article
The restriction enzyme HindIII produces sticky ends. The recognition site is a palindrome having the sequence AAGCTT. It cuts the DNA between the... View Article
HaeIII leaves blunt ends as it cuts both the DNA strands at the same location. Further reading: Plasmid What Is EcoR1? MCQs On Lac Operon... View Article
EcoRI leaves sticky ends with AATT overhangs. Further reading: Plasmid What Is EcoR1? MCQs On Lac Operon Genetically Engineered Insulin... View Article
Sticky ends have single strand overhangs, blunt ends do not have single strand overhangs, it terminates in a base pair. Further reading:... View Article
Sticky ends are better than blunt ends because they facilitate ligation by DNA ligase by forming hydrogen bonds between complementary bases of... View Article
DNA fragments cut by restriction enzymes are called restriction fragments. They can be separated by gel electrophoresis. Further reading:... View Article
Restriction enzymes are known as molecular scissors. They are endonucleases and cut the DNA at specific points. Further reading: Plasmid... View Article
Restriction sites have a specific 4-8 nucleotide long sequence which is recognised by the restriction enzymes. Restriction enzymes cut DNA within... View Article
The recognition site for EcoRI is a palindrome. It is a six nucleotide sequence containing GAATTC in 5’ to 3’ direction and the complementary... View Article
