Recognition site is a 4-8 nucleotide long sequence present on DNA, which is recognised by the restriction enzyme. Further reading: Plasmid... View Article
Recognition site is the sequence of bases that are identified by restriction enzymes for cutting DNA at a specific site. Further reading:... View Article
DNA is negatively charged so it moves towards the anode. The DNA fragments separate according to their size. The smallest fragment moves the... View Article
pUC plasmid is a cloning vector created by modifying pBR322. UC stands for the University of California, where it was synthesised. It is smaller... View Article
pUC18 is a plasmid cloning vector. It was synthesised at the University of California. Yanisch-Perron et al created pUC18. Further reading:... View Article
pUC18 is a plasmid cloning vector. It consists of 2686 bp. It is commonly used in E. coli. Further reading: Plasmid What Is EcoR1? MCQs... View Article
When foreign DNA is inserted into the gene coding for an enzyme, it results in the inactivation of the gene, which synthesises the enzyme. It is... View Article
The term plasmid was coined by J Lederberg in 1952 to describe extrachromosomal genetic material. Further reading: Plasmid What Is EcoR1?... View Article
The first cloning vector was produced by Herbert Boyer and Stanley Cohen. It was a plasmid vector called pSC101. Further reading: Plasmid... View Article
pBR322 is a useful vector because it contains antibiotic resistance genes as a selectable marker and recognition sites for the action of... View Article
Reporter gene is a gene that is attached to the regulatory sequence to be studied. It imparts a specific characteristic, which can easily be... View Article
Cloning site is the site where DNA is inserted into the vector. Restriction enzyme recognises the recognition site and cuts the DNA for insertion... View Article
