Question

In the chromatography technique, the purification of a compound is independent of:

• A. Length of the column or TLC plate
• B. Mobility or flow of solvent system
• C. Physical state of the pure compound
• D. Solubility of the compound

Answer 1

The correct option is C

Physical state of the pure compound

Correct option is C:

Explanation of correct option:

1. Chromatography is based on the phenomenon of adsorption.
2. It does not depend upon physical state of pure substance.

Explanation of incorrect option:

Option A:

1. Instead of spreading a thin layer of the stationary phase on a plate, the solid is packed into a long, glass column either as a powder or a slurry.
2. Sometimes these columns are several inches wide and a few feet long.
3. A large amount of material can be purified on a chromatography column.

Option B:

1. Ascending paper chromatography: The solvent moves in an upward direction.
2. Descending paper Chromatography: It is called descending because the flow of solvent is downwards due to gravitational pull.

Option D:

1. Solubility does not affect chromatography.
2. Differences in intermolecular forces affect chromatography.
3. In paper chromatography, for example, you dissolve the components of a mixture in a solvent.
4. As the solution moves along the paper, the various constituents travel at different speeds.

Final answer: So, it doesn't depend on the physical state of the pure compound.