Question

Bioassay of Auxin is:

• A. Callus formation
• B. Avena curvature test
• C. Seed dormancy
• D. Culture of fungus

Answer 1

The correct option is B

Avena curvature test

The correct option is B.

Explanation for the correct option:

1. The 1928 investigations conducted by Went served as the foundation for the Avena Curvature Test.
2. Auxin up to 300 pg/litre can be measured using this test.
3. Auxin is synthesized in the tissues of SAM and RAM.
4. There are two different types of apical meristem tissue: root apical meristem (RAM), which provides the meristematic cells for future root growth, and shoot apical meristem (SAM), which gives rise to organs like the leaves and flowers.
5. In the Avena curvature test, the SAM is cut and a block of agar containing synthesis auxin is kept onto it.
6. The auxin synthesis increases and the coleoptile starts showing curved movement.
7. This test measures the degree of curvature in oat coleoptiles.
8. The Avena curvature test is used to determine the biological activity of auxins.
9. From the shoot tip to the area of elongation, auxin travels.
10. Auxin's movement is constantly polar.

Final answer: Bioassay of Auxin is Avena curvature test.