Question

Does Reverse Transcriptase need a Primer?

Answer 1

Reverse Transcriptase:

1. A reverse transcriptase (RT) is an enzyme that converts an RNA template into complementary DNA (cDNA).
2. A primer is a brief sequence of nucleic acids that serve as the beginning point for DNA synthesis.
3. A short DNA oligonucleotide known as a primer is required for reverse transcriptases.
4. tRNA acts as a primer in retroviral reverse transcription by binding to the primer binding site.

Significance of primer in reverse transcriptase

1. To attach the RNA template and act as a starting point for strand synthesis