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B
High temperature
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C
Substrate concentration
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D
All of these
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Solution
The correct option is D
All of these
The correct option is D.
Explanation for correct options:
Option A:
Enzymes are known to be affected by changes in pH.
The most favorable pH is the one where the enzyme shows the highest activity.
Enzymes get denatured at extreme temperatures and pH and lose their ability to bind to substrates.
Each enzyme has a specific optimum temperature and pH at which it shows the maximum activity, e.g. optimum pH for pepsin is 1.8, whereas for salivary amylase it is 6.8.
Option B:
Generally, with an increase in temperature, enzyme activity also increases.
Each enzyme is known to show maximum activity at a specific optimum temperature.
The activity is known to decline above and below the optimum temperature.
Option C:
Enzyme activity is known to increase with an increase in substrate concentration.
Enzyme activity only increases till the point of saturation in presence of substrate.
Final answer: Enzyme activity is influenced by pH, high temperature, and substrate concentration.