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Question

Give one means of requiring the ability to detect DNA.


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Solution

Means of detecting DNA:

  1. The ability to detect DNA is required for recombinant DNA methods.
  2. The absorption of ultraviolet light is one approach to determining the amount of RNA or DNA in a solution.
  3. As the ring structures in the bases absorb UV light, the DNA also absorbs it.
  4. Ultraviolet light is also absorbed by single-stranded RNA and free nucleotides. They absorb more light owing to their loose structures.
  5. The amount of absorption is equated with a set of known parameters that allow for determining the DNA concentration.

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