Question

How are transformants selected from non-transformants?

Answer 1

Transformants:

1. Transformants are cells that contain foreign genetic material.
2. Transformants are selected from non-transformants by the presence of a selectable marker, e.g. transformants can be selected by plating them on the antibiotic-containing medium, for the antibiotic resistance gene present in the plasmid.
3. Transformants are selected from non-transformants by the presence of the antibiotic resistance gene that gets inactivated due to the insertion of alien DNA.